Table 1.

Primers Used in PCR-SSCP Assay of Fas Gene

Primer (site of PCR)	Sequence	Size of PCR product (bp)	Annealing temperature (°C)
PA-F (promoter)	5′-ccatcctccttatcccacttcttt-3′
		124	60
PA-R	5′-gcttgtctctgttccacctttca-3′
PB-F (promoter)	5′-ggcgcaacatctgtactttttcat-3′
		145	58
PB-R	5′-agccttggctaattgctggagt-3′
1-F (exon 1)	5′-ctcttctcccgcgggttggt-3′
		171	61
1-R	5′-cactttgcctatccccgggactaa-3′
2-F (exon 2)	5′-gttgcttacttcagaaatcaataa-3′
		249	53
2-R	5′-actgtaatctctggatgttttgt-3′
3A-F (exon 3)	5′-acttcccaccctgttacctg-3′
		192	60
3A-R	5′-catcacacaatctacatcttctgc-3′
3B-F (exon 3)	5′-gtacacagacaaagcccatttttc-3′
		171	57
3B-R	5′-gtgtcaacatagcaccacagtagg-3′
4-F (exon 4)	5′-cgcgataactaatagtttccaa-3′
		228	55
4-R	5′-ctctcagtcagtgttacttcccta-5′
5-F (exon 5)	5′-aattattctgccaggcttttg-3′
		180	53
5-R	5′-gattggtttttcttcacatctttc-3′
6-F (exon 6)	5′-tttcatataatatgccaatgttcc-3′
		145	60
6-R	5′-cttcccccaagttatttcaat-3′
7-F (exon 7)	5′-ctacaaggctgagacctgagtt-3′
		164	55
7-R	5′-tttcaaggaaagctgatacctatt-3′
8-F (exon 8)	5′-ttgtctttctctgcttccatt-3′
		117	53
8-R	5′-attggcctattactctaaaggatg-3′
9A-F (exon 9)	5′-tgctggagtcatgacactaagt-3′
		163	50
9A-R	5′-caatgtgtcatacgcttctttc-3′
9B-F (exon 9)	5′-taattggcatcaacttcat-3′
		175	49
9B-R	5′-gaatttgttgtttttcactcta-3′
9C-F (exon 9)	5′-ggttttcactaatgggaatttcat-3′
		191	50
9C-R	5′-cttcattgacaccattctttcg-3′

F, forward primer; R, reverse primer.