Skip to main content
. 1999 Jun;154(6):1857–1866. doi: 10.1016/S0002-9440(10)65443-0

Figure 4.

Figure 4.

Case 1. A: CDRIII PCR of microdissected tissue obtained from a CD5-stained section. Lane mw: Molecular weight standard. Lanes 1 and 4: CD5+ MCL compartment. lanes 2 and 3: CD5-FL compartment. Lane n: negative control. Lane p: polyclonal control (microdissected reactive follicle). In addition to a weak band of the same size as the MCL compartment, the FL lanes show a second band of different size. Each lane represents a separate microdissection. B: Southern blot of bcl-2 PCR products obtained from paired microdissections with ∼30,000 cells (Lanes 1 and 2), 15,000 cells (Lanes 3 and 4), and 5000 cells (Lanes 5 and 6). Lanes 1, 3, and 5 show results obtained from the MCL compartment and Lanes 2, 4, and 6 those from the FL compartment. Lane w: PCR product from whole tissue section. Lane p: positive control. The weak signal in Lane 1 probably results from contaminating FL lymphoma cells.