Figure 1.
Repression of SER3 is strongly dependent on Snf2. (A) Northern analysis of SER3, Ty1, and TPI1 mRNAs. RNA was isolated from wild-type (FY2082), snf2Δ (FY2083), snf2-798 (FY2084), swi1Δ (FY1852), swp73Δ (FY1702), snf5Δ (FY1658), snf6Δ (FY2085), snf11Δ (FY2086), swp29Δ (FY2087), and swi3Δ (FY2088) strains grown in YPD to 1–2 × 107 cells/mL. (B) Quantitation of Northern analysis. The relative levels of the SER3 (top bar graph) and Ty1 (bottom bar graph) mRNAs were measured by PhosphorImager (Molecular Dynamics) and normalized to the level of TPI1 mRNA. The level of SER3 mRNA in the snf2Δ strain and Ty1 mRNA in the wild-type strain were set to 100. Each value represents the average and standard error of at least three independent experiments.