. 1999 Jun;154(6):1911–1921. doi: 10.1016/S0002-9440(10)65449-1

Table 1.

BAL Analysis of Protein and Leukocytes in Control, Acute-OVA, and Chronic-OVA Challenged Mice

Exposure	Protein (μg/ml)	Total leukocytes × 10⁴	Macrophages × 10⁴ (%)	Eosinophils × 10⁴ (%)	Lymphocytes × 10⁴ (%)
Control (unexposed)	130 ± 10	3.7 ± 0.6	3.7 ± 0.6	0	0
			(>98)	(<1)	(<1)
Acute
1 day	155 ± 21	5.3 ± 0.4	5.1 ± 0.5	0	0
			(96 ± 2)	(<1)	(<1)
3 days	136 ± 18	13 ± 2^*	6.5 ± 1.0^*	5 ± 2^*	1.3 ± 0.3
			(54 ± 12^*)	(34 ± 10^*)	(10 ± 2^*)
5 days	232 ± 25^*	48 ± 9^*	7.0 ± 5.0^*	30 ± 5^*	9.4 ± 2.0^*
			(20 ± 5^*)	(60 ± 7^*)	(19 ± 2^*)
7 days	305 ± 89^*	132 ± 7^*	13 ± 2^*	91 ± 9^*	28 ± 5^*
			(10 ± 1^*)	(68 ± 4^*)	(21 ± 5^*)
10 days	280 ± 17^*	147 ± 10^*	11 ± 5^*	106 ± 20^*	32 ± 10^*
			(8 ± 2^*)	(73 ± 2^*)	(19 ± 3^*)
Chronic
3 weeks	203 ± 29^*	124 ± 16^*	56 ± 11^*	28 ± 9^*	39 ± 13^*
			(45 ± 10^*)	(22 ± 6^*)	(31 ± 5^*)
6 weeks	178 ± 19	6.4 ± 1.7	4.5 ± 1.0	0	1.9 ± 1^*
			(71 ± 2^*)	(<1)	(29 ± 1^*)

For acute exposure, as described in Materials and Methods, mice were immunized with i.p. OVA/alum weekly for 3 weeks and were then exposed to aerosolized OVA for 1 to 10 days. Twenty-four hours after the last aerosol OVA challenge, mice were sacrificed. For chronic exposure, mice were immunized with 3 weekly i.p. OVA/alum injections as above and then were exposed to aerosolized OVA 5 days/week for 3 to 6 weeks. Twenty-four hours after the last aerosol OVA challenge, mice were sacrificed. BAL cells were counted, and cytospin preparations were stained to obtain the differential leukocyte count. Data represent mean ± SEM values; n = 4 to 8 mice/time point.

*P < 0.05 versus control mice.