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. 1999 Jul;155(1):123–133. doi: 10.1016/S0002-9440(10)65107-3

Figure 6.

Figure 6.

SDS-PAGE of cell layers harvested from amyloid-laden cultures showing C-terminal cleavage of SAA. Cultures were rinsed with RPMI, scraped into PBS, pelleted by centrifugation, and solubilized in SDS gel-loading buffer. A: Coomassie blue-stained PVDF membrane. Lane 1: human AA protein isolated from spleen. Lane 2: Cell layer with amyloid (14-day culture). Lane 3: purified rSAA2. B: Immunoblot of A, probed with anti-mouse SAA antiserum. Lane 1: cell layer containing SAA and AA peptides (dimers of SAA and AA are also present). Lane 2: Purified rSAA2. C: Coomassie blue-stained PVDF membrane. Lane 1: Human AA protein from spleen. Lane 2: Cell layer with amyloid (19-day culture). Thin lines at right indicate peptides subjected to N-terminal sequencing.