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Normal hairpin resolution in Rad50S/S thymocytes. Hairpin coding ends (CE), oligonucleotides were ligated to the D2/J1 blunt signal ends (SE) and the open CEs. Ends were PCR amplified using Dδ2– and oligospecific primers (arrows), and products were detected using the probe shown (line with a circle). Expected sizes of PCR products are indicated. Blot, LMPCR of 1 μg of MBN-treated thymic DNA from scid and Rad50S/S mice. M, radiolabeled size marker; sizes (bp) shown on left.
