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. 1999 Jul;155(1):235–245. doi: 10.1016/S0002-9440(10)65117-6

Figure 1.

Figure 1.

[14C]AA metabolism in benign prostate tissue, showing prominent formation of [14C]15-HETE. A: Reverse-phase HPLC analysis using a Beckman Ultrasphere 5-μm ODS column (25 × 0.46 cm) with a solvent of methanol/water/glacial acetic acid (75:25:0.01, by volume) at a flow rate of 1.01 ml/minute switched to 100% methanol at 40 minutes; 0.5-minute fractions were collected and subjected to scintillation counting. Arrows show retention times of unlabeled HETE standards co-injected with the 14C sample. The experiment shown is representative of eight individual benign specimens analyzed. B: Normal-phase HPLC analysis of fractions 63 through 66 (31.5 to 33 minutes) from reverse-phase analysis in A, using a Beckman Ultrasphere 5-μm silica column (25 × 0.46 cm), a solvent system of hexane/isopropanol/glacial acetic acid (100:1:0.1, by volume) with a flow rate of 1.1 ml/minute and on-line detection of radiolabeled products using a Packard Flo-One Radiomatic detector. Arrows indicate retention times of co-injected unlabeled HETE standards.