Figure 6.

RT-PCR for estrogen receptors in human cholangiocarcinoma cells. The mRNAs of Fas-negative, Fas-positive cholangiocarcinoma cells and MCF-7 breast cancer cells as a positive control were isolated using RNAzon kit and RT-PCR for the estrogen receptor was performed as described in Materials and Methods. PCR products were electrophoresed on 1% agarose gel and stained with ethidium bromide. Lane 1 is DNA marker. Lane 2 represents the positive control MCF-7 breast cancer cells. Lane 3 represents Fas-negative cells. Lane 4 represents Fas-positive human cholangiocarcinoma cells. Migration of GPDH housekeeping products (450 bp) and estrogen products (350 bp) are labeled.