Skip to main content
. 2002 Sep 1;16(17):2219–2224. doi: 10.1101/gad.232502

Figure 1.

Figure 1

Kinetics of LPS-induced gene expression in human monocyte-derived DCs. (A) Steady-state mRNA levels of LPS-induced genes were analyzed by RT-PCR. Dendritic cells were stimulated with 100 ng/ml LPS as indicated. (B) RNApolII recruitment to the same genes was analyzed by Chromatin Immunoprecipitation (ChIP) assay using an antibody directed against the RNApolII large subunit Rpb1. Immunoprecipitated DNA was analyzed by PCR using promoter-specific primers. The peak of RNApolII recruitment to the IkBα, IL-8 and MIP-1α genes occurs at earlier time points (0.5–2 h) than those shown in the figure. (C) Promoter-specific histone H3 and H4 acetylation was investigated by ChIP assays carried out with antibodies against acetylated H3 (Ac-Lys 9/Lys 14) and H4. The antibody against hyperacetylated H4 has a preference towards tetra- and tri-acetylated H4.