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. 1999 Aug;155(2):493–503. doi: 10.1016/S0002-9440(10)65145-0

Figure 1.

Figure 1.

Immunostaining specific for procollagen type III (A, B), tenascin (C, D), and undulin (E, F). Procollagen type III specific staining is present in the lamina propria of normal mucosa, particularly underneath the superficial and crypt epithelium (A), and accumulates in the superficial collagenous layer of CC (B). Tenascin-specific staining is largely localized to a thin subepithelial layer in beneath the surface epithelium of normal colon mucosa (C) and stains the subepithelial matrix deposits of CC in their full thickness (D). Tenascin staining is virtually absent from the pericryptal area in CC and normal mucosa. In normal mucosa, undulin is found in small amounts along vessels in the lamina propria and decorating dense fibrillar bundles of the submucosal stroma (E). At variance with tenascin, undulin is absent from the subepithelial collagenous layer of CC (F). Combined immunohistology and in situ hybridization for smooth-muscle α-actin and procollagen α1(I) RNA in CC (cryostat sections; G, H). A proportion of the procollagen α1(I) RNA-positive cells underneath the superficial collagenous band is decorated by α-actin (arrows, CC case no. 8, Table 1 ▶ ). APAAP technique, original magnification, ×100.