Figure 1.

Detection of Igκ gene rearrangement. Genomic DNA from human B cell lines (lane 1, CB33; lane 2, RD), reactive tonsil (RT) (lane 3), and patient samples (CLL, MCL, FCL, MZBCL, RLP) was subjected to PCR amplification of FR3Jκ regions and analyzed with 3% agarose gel (A), ndPAGE (B), and SSCP (C). A: Polyclonal and monoclonal patterns were not discriminated on 3% agarose gel and appeared as single bands. Instead, the polyclonal and monoclonal populations were well separated on 6% ndPAGE. B: The polyclonal pattern appears as a clear smear, and the monoclonal pattern shows a distinct single band (approximately at 120 bp). C: The clonality detected using ndPAGE/gel was confirmed by SSCP analyses.