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Effect of RGD peptides on the protective effect of collagen IV and laminin. Rat mesangial cells were subcultured onto (A) collagen IV (100 μg/ml) and (B) laminin (100 μg/ml) coated wells in full medium plus increasing concentrations of RGD peptides as shown. After 16 hours, they were washed 3 times, and incubated in RPMI plus no additives for 8 hours and the number of cells undergoing apoptosis was counted. Mean ± SE, n = 3.
