RNA and protein expression of Tc-tll. Embryos were stained either with a Tc-tll-specific antibody (a–d) or by whole-mount in situ hybridization (e, f). a–f represent progressively older stages of embryogenesis. Anterior is to the left, a, b, and d are lateral views (dorsal is up), and c, e, and f are ventral views. No staining is detected in the growth zone (gz) with the beginning of germband growth (d and e), whereas the expression in the head (h) persists. See text for further details.