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. 1999 Sep;155(3):915–925. doi: 10.1016/S0002-9440(10)65191-7

Figure 4.

Figure 4.

T-cell repertoire diversity and clonal expansions in PBL from Churg-Strauss syndrome patient LM. Using the Immunoscope RT-PCR technique, TCRVβ chain segments in PBL from patient LM was amplified using a panel of 24 Vβ subfamily-specific PCR primers. Clonal expansions was detected in several Vβ-Cβ profiles. Clonality of Vβ12- and Vβ21-specific transcripts is shown here. Samples were analyzed from the peripheral blood prior to (PBL before) and after immunosuppressive treatment (PBL after). Clonal expansions are indicated by arrows. Clonality of the Vβ-Cβ profiles was confirmed using Jβ-specific primers. For both Vβ-Cβ profiles, each of 13 Vβ-Jβ recombinations was selected to demonstrate the strongest deviation from normal polyclonal gaussian-like peak size distribution, ie, Vβ12-Jβ2.2 for the Vβ12 family and Vβ21-Jβ1.2 for the Vβ21 family.