Figure 5.

Sensitivity of PBL to CD95-mediated apoptosis. Lymphocytes were isolated from the peripheral blood from four healthy donors (filled circles) and from five CSS-patients (open circles). After treatment with PHA (2.4 μg/ml) for 24 hours, the lymphocytes were incubated with an agonistic anti-CD95 antibody at various concentrations. After another 24 hours, the lymphocytes were subjected to TUNEL analysis as described in Materials and Methods and percentages of apoptotic lymphocytes were counted. Data are given as means ± SE.