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. 1999 Sep;155(3):915–925. doi: 10.1016/S0002-9440(10)65191-7

Figure 6.

Figure 6.

Distribution of CD95 receptor isoform mRNA expression in peripheral blood T cells (PBL), gastric intraepithelial T cells (IEL) and eosinophils. A: cDNAs derived from PBL of one healthy donor, patient WI 1 day prior to (CSS, day −1) and 14 days after gastrectomy and immunosuppressive treatment (CSS, day 14) were amplified using CD95 isoform- and HPRT-specific primers as described in Materials and Methods in 30 PCR cycles. Amplification products for membrane-bound CD95 (CD95Tm, 392 bp), soluble CD95 (CD95Sol, 330 bp), and HPRT (237 bp) were separated on a 2% agarose gel. B: CD95 membrane-bound and soluble isoform mRNA expression was analyzed in eosinophils from healthy donors (representative for three donors), one patient with infectious eosinophilia (patient DA, 25% eosinophilic counts), and one CSS patient (patient PF, 32% eosinophilic counts). C: CD95 isoform mRNA expression in ulcerative and non-ulcerative gastric mucosa was studied in samples from gastric antrum and corpus of patient WI at the time of gastrectomy.