Figure 7.

Identification of T cells as CD95L-expressing cells in ulcerative gastric mucosa in patient WI. Cryosections from ulcerative gastric mucosa from patient WI were double-stained using CD3-specific and CD95L-specific antibodies as described in Materials and Methods. In A and B, one cryosection from infiltrated muscularis propria and in C and D one from infiltrated mucosa is shown. Double fluorescence staining for CD3 and CD95L was performed on the same cryosection. In A and C, signals are specific for CD3 (FITC-labeled), in B and D, for CD95L expression (CY3-labeled). CD3-expressing cells (ie, T lymphocytes) and CD95L expression were colocalized (arrows).