Table 2.
Sequence Analysis of TCRβ-VDJ Junctions in CSS Patients
| Patient | Junctional Sequence | ||
|---|---|---|---|
| Vβ21 | D, N | Jβ 1.2 | |
| VN | CASS | FAF | YGYT |
| WI | CASS | FGT | YGYT |
| GM | CASS | AGT | YGYT |
| PF | CASS | SGT | YGYT |
| LM | CASS | SAT | YGYT |
| WI* | CASS | FSAT | YGYT |
| SJ | CASS | LGT | YGYT |
| PF* | CASS | LQAGS | GYT |
| SJ* | CASS | LQAA | YGYT |
Transcripts from rearranged TCRβ VDJ genes were amplified from cDNAs derived from PBL of eight CSS patients using Vβ21- and Jβ1.2-specific primers. With the exception of cDNAs from patients GG and NB, the sequence of the TCRβ VDJ-junctions were readable in six patients with a CDR3 of 11 aa in length. Protein sequence information is given as single letter amino acids derived from the 3′ end of the rearranged Vβ21.3 gene (Vβ21), the germline encoded D-region, the N-region generated during VDJ-recombination (D,N), and the 5′ end of the rearranged Jβ1.2 gene (Jβ1.2). Amino acids matching the S〈G/A〉T motif are underlined. Those fitting the LQA motif are in bold type.
*Sequences that correspond to additional TCRβ VDJ-rearrangements with a CDR3 length of 12aa amplified from patients PF, SJ and WI.