Table 5.

PBL from Healthy Donors Are Rescued from CD95-Mediated Apoptosis by Supernatants from Cultured CSS-PBL

	Apoptotic lymphocytes [%]
Control	89 ± 4
CSS-Sn	66 ± 7*
CSS-Sn+ neutralizing anti-CD95	90 ± 6

T lymphocytes from three healthy volunteers were isolated from the peripheral blood and incubated either in RPMI medium (control) or with a mixture of supernatants which have been derived from cultured lymphocytes from three CSS-patients (CSS-Sn). After 24 hours, lymphocytes were treated with 100 ng/ml of an agonistic anti-CD95 antibody for another 24 hours and subjected to TUNEL analysis. In some experiments, the supernatant mixture was pretreated with a neutralizing anti-CD95 antibody at 1 μg/ml (neutralizing anti-CD95). Data are from three independent experiments and given as means ± SE.

*Significantly different from the control (P < 0.05).