Table 1.
Summary of Antibodies to τ/PHFτ
| Antibody | Specificity | Type | Dilution |
|---|---|---|---|
| AT8 | phos Ser202, Thr205 | M | 1:500 |
| PHF1 | phos Ser396, Ser404 | M | 1:2000 |
| AP422 | phos Ser422 | P | 1:1000 |
| T14 | p-ind τ 141–178 | M | 1:1000 |
| T46 | p-ind τ 404–441 | M | 1:1000 |
| Tau1 | dephos Ser202 | M | 1:2000 |
AT8, PHF1 and AP422 are phosphorylation-dependent antibodies to τ that recognize PHFτ, but not enzymatically dephosphorylated PHFτ as well as autopsy-derived adult human CNS τ. T14 and T46 are phosphorylation-independent antibodies to τ that recognize adult human CNS τ as well as PHFτ and its dephosphorylated preparation. Tau1 is a dephosphorylation-dependent antibody to τ that recognizes normal CNS τ, and it only binds to PHFτ after enzymatic dephosphorylation. The numbering system for the amino acid sequences of the epitopes in τ recognized by the anti-τ antibodies listed here is based on the largest human CNS τ isoform. 51
phos, phosphorylated; Ser, serine; Thr, threonine; p-ind, phosphorylation-independent; dephos, dephosphorylated; M, monoclonal antibody; P, polyclonal antibody.