Figure 5.

Serum-inducible SMC proliferation is inhibited by antisense Egr-1 PS-ODNs. SMCs were exposed to 1 μmol of PS-ODN 6 hours after the change of media to serum-free. Medium containing serum (5% FBS) and 1 μmol/L of PS-ODN E1, AS2, E11, AS2C, E11C (A) or E11, E11C, E11M, E11S (B) was added 18 hours subsequently and incubated for a further 72 hours. Cell numbers were quantitated as described under “Materials and Methods.” The data are representative of at least two independent experiments performed in triplicate. Standard errors of the mean are indicated in the figure. **, P < 0.01 (Student’s paired t-test) compared to control (No ODN).