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Antisense Egr-1 PS-ODNs inhibit the induction of Egr-1 in SMCs exposed to serum. Western blot analysis was performed with antibodies to Egr-1 and Sp1, and growth-arrested SMCs exposed to 1 μmol/L of E1, E11, and AS2 for 24 hours before the addition of serum and incubation for 1.5 hours, as described under “Materials and Methods.” The bands were quantitated using a scanning densitometer and Egr-1 values normalized to Sp1. The blot is representative of three independent experiments.
