Figure 4.

Dimeric and multimeric forms of VAP-1 are present in smooth muscle cells. [³⁵S]Methionine/cysteine-labeled fragments of smooth muscle were precipitated with an anti-VAP-1 mAb 1B2 and with a negative control mAb 3G6. Under reducing conditions, the monomeric 90–100-kd form of VAP-1 (thick arrow) and the dimeric VAP-1 (arrowhead) are most prominent. Fainter but specific VAP-1 bands are also visible at higher molecular mass (thin arrows). Molecular mass standards in kd are shown on the right.