Figure 5.

VAP-1 dimer in smooth muscle is smaller than that in endothelial cells. Lysates from the indicated tissues were prepared, separated in SDS-PAGE under nonreducing conditions, and subjected to immunoblotting with an anti-VAP-1 mAb 1B2 and with a negative control mAb 3G6. Samples from skeletal muscle, heart, and tonsil contain mostly endothelial VAP-1, whereas vessel and gut samples are of smooth muscle origin. All samples were analyzed in the same gel, which is a representative of three independent analyses. Note that the negative control was exposed 4 times longer than the 1B2 filter.