Figure 2.

Immunohistochemical and in situ hybridization analysis of the cellular distribution and patterns of expression of psoriasin within psoriatic skin and breast carcinoma. Psoriasin protein is localized in hyperplastic epidermis of skin to both nuclei (A, white arrow) and cytoplasm (A, black arrow) of keratinocytes. Similar nuclear and cytoplasmic staining is seen in breast epithelial tumor cells (C, black arrow; case 8965). Psoriasin protein is also detected within occasional stromal inflammatory cells (C, white arrow). E: H&E-stained section from the same region of the tumor shown in C. Psoriasin mRNA expression in skin is restricted to epithelial cells in suprabasal layers of epidermis (B) and scattered invasive epithelial tumor cells in breast tumors (D), detected using antisense probe (B and D) compared to sense probe (F). Original magnification for all panels at the microscope, ×200.