Figure 2.

Virtual Northern blot analysis of mitochondrial components, cytochrome c oxidase 1 and cytochrome b, ribosomal protein L3, and β-actin. PCR-generated full-length cDNAs from human placenta, HUVEC and HEVECs cultured in vitro for 2 days (HEVEC-2D; 98% MECA-79-positive cells) or 8 days (HEVEC-8D; <1% MECA-79-positive cells) were electrophoresed on a 1% agarose gel, transferred to nylon filters, and hybridized under high-stringency conditions with ³²P-labeled cytochrome c oxidase 1 (A), cytochrome b (B), ribosomal protein L3 (C), and β-actin (D) cDNA probes.