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. 1999 Dec;155(6):2043–2055. doi: 10.1016/S0002-9440(10)65523-X

Figure 3.

Figure 3.

Immunofluorescence staining of MECA-79-positive HEVs with antibodies to thrombospondin-1, mac25/IGFBP-rP1, and DARC. A–C: Antibody to thrombospondin-1 stains the HEV basal lamina. Cryosections of human tonsils (4 μm, fixed with acetone) were double-stained with mouse MAb P10 against human thrombospondin-1 (A) and HEV-specific rat MAb MECA-79 (B). White arrows indicate HEVs coexpressing MECA-79 antigens and thrombospondin-1. Two-color staining (C) with monoclonal antibodies P10 (green) and MECA-79 (red) reveals that thrombospondin-1 accumulates in the basal lamina of MECA-79-positive HEVs (arrowheads). D–F: Association of the secreted matricellular protein mac25/IGFBP-rP1 with the surface of MECA-79-positive HEVECs in situ. Cryosections of human tonsils were double-stained with rabbit antibodies against human mac25/IGFBP-rP1 (D) and MAb MECA-79 (E). White arrows indicate HEVs coexpressing MECA-79 antigens and mac25. Two-color staining (F) with anti-mac25/IGFBP-rP1 (green) and MECA-79 (red) antibodies reveals that mac25/IGFBP-rP1 is associated with the lumenal and basolateral surfaces of MECA-79-positive HEVECs (arrowheads). G–I: HEV-specific expression of the promiscuous chemokine receptor DARC in human tonsils. Cryosections of human tonsils were double-stained with mouse MAb Fy6 to human DARC (G) and rat MAb MECA-79 (H). Two-color staining (I) with Fy6 (green) and MECA-79 (red) antibodies reveals that DARC is associated with the surface of MECA-79-positive HEVECs. Original magnifications: A, B, D, E, G, H, ×100; C, F, I, ×1000.

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