Figure 4.

The secreted matricellular protein mac25/IGFBP-rP1 is expressed in HEVECs but not in HUVECs. A: Virtual Northern blot analysis of placenta, HUVEC, HEVEC-2D, and HEVEC-8D cDNAs with a ³²P-labeled human mac25/IGFBP-rP1 cDNA probe. The blot was prepared as described in Figure 2 ▶ . After hybridization with the mac25 probe, the blot was deshybridized and reprobed with a human β-actin cDNA probe. B: Western blot analysis of HEVEC or HUVEC conditioned media with anti-mac25/IGFBP-rP1 rabbit polyclonal antibodies. Molecular weight markers are indicated on the right.