Figure 6.

Virtual Northern blot analysis of placenta, HUVEC, HEVEC-2D, and HEVEC-8D cDNAs with a probe encoding promiscuous chemokine receptor DARC. PCR-generated full-length cDNAs from human placenta, HUVEC and HEVECs cultured in vitro for 2 days (HEVEC-2D; 98% MECA-79-positive cells) or 8 days (HEVEC-8D; <1% MECA-79-positive cells) were electrophoresed on a 1% agarose gel, transferred to nylon filters, and hybridized under high-stringency conditions with a ³²P-labeled human DARC cDNA probe. The blot was standardized for the amount of total cDNA in the indivivual samples with a human β-actin cDNA probe.