Table 3.
Competitive Repopulation between Freshly Isolated Hepatocytes versus Serially Transplanted Hepatocytes
| Rounds of transplantation | Donor cell genotypes* | Ratio | Individual % repopulation† | Average % repopulation‡ |
|---|---|---|---|---|
| 2 | ROSA/Dopa | 1:3 | 45, 48 | |
| 2 | ROSA/WT | 1:1 | 50 | |
| 2 | Dopa/ROSA | 3:1 | 71, 73 | |
| 3 | ROSA/Dopa | 1:3 | 38, 39, 42 | 40 ± 2 |
| 65, 62, 70, 60, 61, | ||||
| 3 | Dopa/ROSA | 3:1 | 65, 54, 58, 60, 69, | 63 ± 5 |
| 62, 71 | ||||
| 3 | ROSA/WT | 4:1 | 65, 65, 70, 70 | 68 ± 3 |
| 7 | ROSA/WT | 1:1 | 46 | |
| 7 | ROSA/WT | 1:4 | 28, 30 |
* The left genotype indicates the cells which had previously been serially transplanted.
† The percentage of repopulation measured in individual mice is given.
‡ Averages of the percentage of repopulation are given with standard deviation when three or more samples were analyzed.