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Western blotting of extracts of brain and spinal cord from wild-type and transgenic htau40 mice of 3 months with phosphorylation-dependent antibodies AT-8, AT-180, and PHF-1. Western blots of brain (a) and spinal cord (b) homogenates of wild-type (WT) and heterozygous htau40-5 (5H) and htau40-1 (1H) transgenic mice. Approximately 100 μg of protein was loaded per lane for incubation with AT-8 and AT-180, and 15 μg for detection with PHF-1.
