Immunogold staining of a dilated axon in the spinal cord of a transgenic htau40-1 mouse (homozygous, 8 month). Preembedding immunogold staining with AT-8 (a) and Alz-50 (b). Note that the silver-enhanced gold particles are equally dispersed in the axoplasm. The irregularity of the particles is due to the silver enhancement and osmium postfixation. Magnification: a, ×25,200; b, ×35,700.