Figure 8.

Cryosection of a normal platelet prepared for study after incubation under hypertonic conditions (see text). The frozen section was incubated first with API and 6DI and then with anti-mouse IgG coupled to 10 nm gold. Hypertonic salt solutions have caused dilatation of the OCS. Gold particles detecting sites of GPIb are evenly dispersed on the exposed surface (S) and membranes lining channels of the OCS. There is no significant variation in the frequency of GPIb on external and internal membranes. Original magnification, ×39,000.