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. 1999 Dec;155(6):2019–2027. doi: 10.1016/S0002-9440(10)65521-6

Figure 4.

Figure 4.

There is neither CD40-ligand nor Fas-ligand protein expression in tumor B cells, as demonstrated by Western blot analyses. CD40-ligand (A) and Fas-ligand protein (B) was detectable in a stimulated C8166 T-cell line (lane 2) as the positive control, and CD40-ligand protein was detectable in a Jurkat T-cell line (lanes 7 and 8). Neither protein expression was found in unstimulated nor in stimulated normal and tumor B cells (lanes 3–6). The cells were stimulated for 24 hours as described in Materials and Methods. Lanes 1 and 2: Cell line C8166 unstimulated and stimulated. Lanes 3 and 4: Purified memory B cells unstimulated and stimulated. Lanes 5 and 6: Tumor B cells (no. 8) unstimulated and stimulated. Lanes 7 and 8: Jurkat cell line, unstimulated and stimulated, respectively.