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. 1999 Nov;155(5):1549–1555. doi: 10.1016/S0002-9440(10)65471-5

Figure 3.

Figure 3.

Overexpression of human cadherin-11 cDNA in osteosarcoma cell lines. Transfection of the intact form of the cadherin-11 gene into the human osteosarcoma cell lines, HOS and Saos-2, was performed with methods described previously. 8 Panels show Western blot analyses of each cell lysate (a) and culture supernatants concentrated 20-fold (b). L cell, Hos, and Saos-2 transfectants with the vector alone (pCXN2) are termed L-mock, Hos-mock, and Saos-2-mock, respectively, and transfectants with the intact cadherin-11 expression vector are termed L-cad-11(i), Hos-cad-11(i), and Saos-2-cad-11(i), respectively. The intact form of cadherin-11 was found as a 120-kd band, and the secreted form was an 80-kd band.