Figure 4.

Immunohistochemistry of cadherin-11, N-cadherin, and β-catenin in normal bone tissues and osteosarcoma. Upper panels show the immunostaining of fetal calvaria in 19 weeks in gestation (a–c) and adult reactive bone (d–f), and lower panels show primary (OS7: g–i) and metastatic (OS12: j–l) osteosarcoma. Monoclonal antibody against cadherin-11(113E) (a, d, g, j), polyclonal rabbit antisera against P,E,N-cadherin (b, e, h, k), and monoclonal antibody against β-catenin (c, f, i, l) were used for immunohistochemistry with peroxidase streptavidin-biotin methods. Original magnification ×400.