Figure 3.

[³H]AA release in GEC. A: [³H]AA-labeled GEC were incubated with anti-GEC antibody and normal human serum (NS) to form C5b-9, or with heat-inactivated serum (HIS) in controls, for 40 minutes at 37°C (*P < 0.01 NS versus HIS, 5 experiments performed in duplicate). B: Antibody-sensitized GEC were incubated with C8DS+C8, C8DS, or heat-inactivated serum for 40 minutes at 37°C. Significant differences were present among groups (ANOVA P < 0.03; **P < 0.02 C8DS+C8 versus C8DS; 3 experiments performed in duplicate). C: GEC were incubated with medium alone, or with EGF 100 ng/ml, bFGF 50 ng/ml, or hepatocyte growth factor (HGF) 25 ng/ml for 60 minutes, or PMA 250 ng/ml for 30 minutes at 37°C. Cells were then permeabilized with medium containing digitonin and 0.2 μmol/L or 1 mmol/L free Ca²⁺ concentration. Significant differences were present among groups (ANOVA P < 0.01; ⁺P < 0.02 EGF, HGF, or PMA versus medium with 1 mmol/L [Ca²⁺]; 3 experiments performed in duplicate). It should be noted that EGF and PMA do not increase free [³H]AA when the free Ca²⁺ concentration is maintained at 0.2 μmol/L. 20