Table 5.

Effect of AG1478 on ERK2 Tyrosine²⁰⁴ Phosphorylation in GEC

Stimulus	AG1478 (nmol/L)	Inhibition (%)	N
Complement	300	62 ± 7*	4
EGF	300	100 ± 1*	4
	30	52 ± 24†	4
	3	0	2
PMA	300	−15 ± 10‡	4

GEC were untreated or were preincubated with AG1478 for 15 minutes at 37°C, then incubated with complement, EGF, or PMA (as in Figure 6 ▶ ). ERK2 tyrosine²⁰⁴ phosphorylation was monitored by immunoblotting and quantitated by densitometry. Inhibition was determined by comparing stimulated increases in ERK2 tyrosine²⁰⁴ phosphorylation with and without AG1478.

*P < 0.0001,

^†P = 0.06 versus untreated.

^‡Negative value indicates a trend toward stimulation in the presence of AG1478.