Figure 1.

Expression of transfected trkA and trkB in SH-SY5Y neuroblastoma cells. Receptor autophosphorylation after NT treatment probed with 4G10 anti-phosphotyrosine antibody. Cells were cultured for 3 days in the presence (1 μg/ml) and absence of tetracycline in RPMI with 10% fetal bovine serum (FBS) and were stimulated for 5 minutes with NGF and BDNF (100 ng/ml). Five hundred micrograms of protein was immunoprecipitated with 1 μg of rabbit polyclonal anti-pan trk antibody (C-14) and was electrophoresed in 8% SDS-PAGE. E2 had approximately the same level of receptor autophosphorylation as the parental SH-SY5Y after NGF treatment. tet +: 1 μg tetracycline/ml; tet −: 0 μg tetracycline/ml.