Figure 1.
Gastric ulceration stimulates Raf-1 kinase activity. Tissue lysates containing equal amounts of protein from normal (sham operated) and ulcerated gastric mucosa were immunoprecipitated with anti-Raf-1 antibody. Immunecomplexes were washed and subjected to in vitro kinase reaction, using [γ-32P]ATP and kinase inactive MEK as a substrate and analyzed by SDS-PAGE and autoradiography as described in Materials and Methods. Data are expressed as the mean ± SD (n = 6); each experiment was performed in triplicate.