Figure 8.

Effect of EGF treatment on PKC activity in a rat gastric epithelial cell line (RGM1). Serum-starved RGM1 cells were treated with EGF (10 ng/ml) for 5 minutes. Cell lysates were used to assess PKC activity, following the procedures described in Materials and Methods. EGF treatment of RGM1 cells does not significantly alter PKC activity when compared to untreated controls. Treatment of gastric epithelial cells with 50 nM PMA for 5 minutes (positive control) significantly increased PKC activity, whereas depletion of PKC by prolonged incubation (24 hours) with PMA (50 nmol/L) significantly decreased PKC activity. Data are expressed as the mean ± SD from three separate experiments performed in triplicate.