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Mutational analysis of phosphorylated amino acid residues. (A) Constructs used to map the phosphorylated residues. (B) Transfected HeLa cells were labeled with [32P]phosphate for 2.5 hr before lysis and immunoprecipitation with PIN.1.1. Cells transfected with Iip35 (lane 1); Iip35(S6A) (lane 2); Iip35(S8A) (lane 3) or Iip35(S6, 8A) (lane 4) were analyzed by SDS/PAGE. Molecular size markers are located to the left.
