Figure 5.

Cell surface staining of Iip35. K44A HeLa cells were supertransfected with different Iip35 constructs together with DR3 and CD8 without (Left) or with (ind, Right) induction of the mutant dynamin. Invariant chain at the cell surface was labeled with mAb Bü45 and analyzed by flow cytometry. Stainings of cells transfected with DR3 and the indicated Iip35 constructs are shown. The negative control represents cells stained with secondary antibody alone. The of level CD8 staining (by using mAb OKT8) was equivalent in all transfectants.