Figure 5.

Expression of p53 and p21waf1/cip1 in the lung. A: Western blot analysis for p53 and p21waf1/cip1 in the wild-type and p53 knockout mice. Expression of p53 and p21waf1/cip1 was observed in the wild-type mice after the treatment, whereas the expression of these proteins was not detected in the p53 knockout mice throughout the experimental period. B and C: Immunohistochemistry for p53 (B) and p21waf1/cip1 (C) in a wild-type mouse at 12 hours after the treatment. Numerous positive nuclei were observed in the bronchial epithelia and alveoli. B, immunofluorescent staining; original magnification, ×400. C, immunoperoxidase staining; original magnification, ×400.