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Distribution kinetics of CVB3 in spleens of normal and BcKO mice. C57BL/6 (A-C, E-G) or BcKO mice (D and H) were infected with CVB3, and spleens were harvested 2 or 3 days after infection. In situ hybridization was carried out using CVB-specific negative-sense RNA probe (ISH, A, B, D-F, and H), or sections were analyzed by immunohistochemistry (IHC, C and G) using B-cell-specific antibody.
