Figure 7.

Histopathology and ISH at 45 days after infection in a BcKO mouse. Heart of a BcKO mouse taken 45 days postinfection and analyzed by histology. A and B: Trichrome stain, in which fibrous tissue appears light blue. The boxed area in A is enlarged in B; original magnification, ×80. C-F: ISH, counterstained with H&E. C and E are adjacent sections, and higher magnifications of identical areas (centered over a vessel) are shown in D and F, respectively. A negative-sense probe to detect CVB genomic-sense RNA resulted in the signal seen in C and D, whereas a positive-sense probe to detect genome-complementary RNA failed to generate a strong signal (E and F). Probes were of comparable specific activity.