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. 1999 Oct;155(4):1075–1085. doi: 10.1016/s0002-9440(10)65210-8

Figure 1.

Figure 1.

Northern blot analysis of genes identified by suppression subtractive hybridization analysis in the AAF/PHx protocol of liver regeneration. Poly(A)+-selected RNA was isolated from snap-frozen normal rat liver (N) and livers from AAF-treated animals 96 hours after initiation of the protocol (AAF) and 7, 8, 10, and 11 days after the partial hepatectomy (AAF/PHx). Five micrograms of mRNA were loaded in each lane. 32P-labeled cDNA probes cloned by suppression subtractive hybridization were used for hybridization. Hybridization with GAPDH and staining with ethidium bromide were used to assess integrity and equal loading of RNA samples.