Figure 2.

Northern blot analysis of genes identified by suppression subtractive hybridization analysis in selected nonparenchymal cell populations isolated from rat liver by pronase perfusion after administration of AAF. Animals were administered 2-AAF at 0 hours and killed for cell isolation and subsequent RNA extraction at the time points indicated. Twenty micrograms of total RNA were loaded in each lane. ³²P-labeled cDNA probes cloned by suppression subtractive hybridization analysis were used for hybridization. Hybridization with GAPDH and staining with ethidium bromide were used to assess integrity and equal loading of RNA samples.