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Northern blot analysis of 5 μg poly(A)+-selected RNA hybridized to 32P-labeled cDNA probes for IL-1β, IFN-γRα, IL-18, and ICE. A 70% PHx was performed and animals sacrificed 0 (normal), 1, 6, 12, 24, 30, 36, 42, 48, 54, and 60 hours after the operation. Hybridization with GAPDH and staining with ethidium bromide were used to assess integrity and equal loading of RNA samples.
