Figure 2.

Western blot analysis comparing the time course of inducible nuclear proteins. Nuclear protein was prepared from BASMC exposed to PMA (100 ng/ml) over time. Approximately 10 μg of protein/lane was resolved by 8% polyacrylamide gel electrophoresis and transferred to a nitrocellulose membrane before immunoblotting with polyclonal antibodies to Egr-1, NAB2, and Sp1. Bound antibodies were detected using a horseradish peroxidase-based chemiluminescence method.